Project research established the nucleic acid inductively coupled plasma emission spectrum precis...

Start Date

09/2007

End Date

11/2010

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Method of Quantitative Analysis for Nucleic Acid by ICP-OES, HR-ICP-MS and Certified Reference Materials of Nucleic Acid

 

Project research established the nucleic acid inductively coupled plasma emission spectrum precise quantitative measurement method, the sample PPM level as the uncertainty of measurement results can reach 1%;  established the nucleic acid high resolution inductively coupled plasma mass spectrum quantitative measurement technology high sensitivity, the sample PPB level as the uncertainty of measurement results to achieve 3% method measuring result. Through the phosphorus element certified reference materials the result can be traceability to the international basic unit (SI).

 

The traceability way is clear and solves problem of based on optical density measurement of nucleic acid quantitative vulnerable to protein sugar etc interference, and traceability way complex, can't traceability to the international basic unit (SI). the technical problem for tasted nucleic acid samples of quantitative measurement and related standard research provides methodology. Project development completed calf thymus DNA content (national level standard certificate Numbers: GBW09801) salmon fine DNA content (GBW09802) 20 bp oligo DNA content (GBW09803) 320 bp oligo DNA content (GBW09804) deoxidizing adenine nucleoside a phosphate content (GBW09805) deoxidizing cytidine monophosphate content (GBW09806) deoxidizing guanosine monophosphate content (GBW09807) thymidine monophosphate content (GBW09808) adenine nucleoside a phosphate content (GBW09809) cytidine monophosphate content (GBW09810) guanosine monophosphate content (GBW09811) uridine a phosphorus, Uridine monophosphate content (GBW09812) hypoxanthine nucleoside a phosphate content (GBW09813) ring adenine nucleoside a phosphate content (GBW09814), 14 kinds of national first level standard standard grading certificate no. card words in 1171 manufacturing measuring instruments license key: countries system scale things 10001106 as standard for the photometric method chromatography mass spectrometry analysis of nucleic acid and nucleotide sample, used for the nucleic acid protein analysis instrument related calibration and laboratory self-calibration solved the nucleic acid nucleotide sample analysis and instrument calibration measurement standard of the lack of technical problems in the application, by the use of units of the highly evaluation and experts of identity, have considerable economic benefits and social benefits, this subject for perfecting our country's food and drug, Clinical nucleic acid detection traceability system and improve our country nucleic acid testing data of the accuracy of comparability provides important technical guarantee and necessary value traceability conditions.