Established highly accurate nucleic acid measurement by...

Start Date

January, 2009

End Date

December, 2011


Related Programs and Projects

Contact Us

Wang Jing

Division of Medical and Biological Measurements   

National Institute of Metrology

Tel: 010-64525061

Key technological research on nucleic acids traceability and transferability of genetically modified(GM) plant

(“11th Five-Year Plan” National Science and Technology Support Program, completed)

Project leader: Wang Jing

Ø Established highly accurate nucleic acid measurement by ultrasonic treatment followed by isotope dilution mass spectrometry (Ultrasonic-IDMS), which provides feasibility for reliable, traceable and accurate quantitative measurements of large size or genomic DNA.

Ø Established inductively coupled plasma optical emission spectrometer(ICP-OES) for genetically modified nucleic acid, determined the traceable frame to SI unit of mass.

Ø Developed digital PCR for GM plants, through which the DNA measurement can trace to a single molecule. The measurement uncertainty is 10%.

Ø Developed GM plasmid DNA reference material for crops including GM rice BT63, Ke Ming Dao 2, Ke Feng 6; GM tomato: HuaFan 1, GM cotton SGK 321 and GM soybean Mon89788.

Ø Developed GM matrix reference material to ensure the GM test comparable and reliable, including GM rice: BT63, Ke Ming Dao 2; GM cotton SGK 321, etc. These GM reference materials has met the need of trade for local and regional industry.

Ø Established an identification method of genetically modified rice and cotton seed candidate for manufacturing GM matrix reference material.